Title: Qualitative and Quantitative Analysis of Xinnaokang Capsule
Abstract: OBJECTIVE To quickly identify the salviae miltiorrhiae,paeoniae rubra,pheretima asiatica,rhizoma,puerariae of the Xinnaokang capsule and determine the content of the peoniflorin.METHODS The radix salviae miltiorrhiae,paeoniae rubra,pheretima asiatica,rhizoma,puerariae were identified with thin layer chromatography(TLC),the content of the peoniflorin was determined by HPLC.C18 column was used in HPLC,taking acetonitrile-Water(17∶83) as the mobile phase;230 nm was the detection wavelength and the flow rate was 1.0 mL·min-1. RESULTS The characteristic speck of preparation was identified with TLC and was not observed in the thin layer chromatogram of the blank control.HPLC method was estabilished and the results showed there was a good linear relationship in range 115.5-577.5 μg·mL-1(r=0.999 9,n=5).The average recovery rate was 100.14%,RSD was 0.1%(n=4).CONCLUSION The method is simple and accurate with good reproducibility and high precision.It can be applied in the qualitative and quantitative analysis of Xinnaokang capsule.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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