Title: DangGuiBuXue Decoction in inhibiting proliferation of human liver cancer SMMC-7721 cells in vitro
Abstract: AIM: To observe the effects of the serum of DangGuiBuXue Decoction (DGBXD) on the proliferation of SMMC 7721. METHODS: 25 rabbits were divided into 5 groups at random and the rabbits sera of different time points were collected after ig 50 mL of Naltrii chlorcid (9 g·L -1 ) or DGBXD with the dosage of 32 g, 16, 8, 4 g·kg -1 . The culture medium of human heptocarinoma cell lines of SMMC 7721 was treated by the rabbits sera. Effects of the sera on inhabiting proliferation of SMMC 7721 were detected by MTT assay. RESULTS: The sera of DGBXD ig inhibited the proliferation of SMMC 7721 dose and time dependent manner. Tmax was 45min after ig and the maximum effects of BGBXD was 0.46±0.05 (32 g·kg -1 , P 0.01), 0.53±0.06 (16 g·kg -1 , P 0.01), 0.55±0.04 (8 g·kg -1 , P 0.05), 0.61±0.03 (4 g·kg -1 , P 0.05) vs control. ED50 was 122 g·kg -1 and T 1/2 was 2.2 h. CONCLUSION: The sera of DangGuiBuXue Decoction can inhibit the proliferation of human liver cancer SMMC 7721 in a dose and time dependent manner.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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