Title: Effect of simvastatin on differentiation of osteoblasts derived from bone marrow stem cells.
Abstract: Objective To study the effect of simvastatin on osteoblast differentiation by using a mouse bone marrow culture system.Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in the cultures. Alkaline phosphatase staining and von Kossa staining were used to assess osteoblast differentiation. Semi-quantitative RT-PCR was perfermod for evaluating the expressions of collagen type I (COL1), akaline phosphatase (ALP), CBFA1, and Osterix on culture days 3 and 5, in the simvastatin-(treated) gron and blank control group.Results Cells in each group were differentiated into osteoblast lineage, as shown by alkaline phosphatase and von Kossa stainings. The expressions of COL1, ALP and CBFA1 in the treatment group was higher than those in the control group.Conclusion Simvastatin enhances the expression of osteoblast-differentiation-related genes.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot