Title: Effects of simvastatin on Cbfa1, Cbfb and Osterix expressions in human bone marrow stromal cells
Abstract: AIM: To study the effects of simvastatin on osteoblastic differentiation and proliferation of human bone marrow stromal cells (hBMSC), and investigate the mechanism of the effects of simvastatin on hBMSC. METHODS: hBMSC derived from patients with femur fracture were cultured in vitro. After sub- culturing, the medium of treatment group were added 10 -7 mol/L simvastatin. Semi-quantitative RT-PCR was performed for evaluat- ing the expressions of Cbfa1, Cbfb and Osterix (OSX) mRNA. Cellular alkaline activity and mineralization were examined by ALP measurement kit and Alizarin Red-S staining. Cell Count Kit-8 (CCK-8) was used to examine proliferation. RESULTS: The expressions of Cbfa1 and OSX mRNA in treatment group were higher than those of the control group at 3, 6, 9, 12 d (P 0.01), but no significant effects were observed on the expression of Cbfb mRNA in both groups (P0.05); The activity of ALP in treatment group was higher than that of the control group at 3 d (54.05±1.14 vs 63.54±2.99, P0.05), 6 d (60.53±3.08 vs 70.39±3.98, P0.01), 9 d (72.93±0.85 vs 86.28± 1.20, P0.01), 12 d (74.13±1.79 vs 94.99±3.20, P 0.01); Simvastatin enhanced osteoblast-mediated mineralization; Compared with control group, the proliferation of hBMSC was inhibited in treatment group (P0.05). CONCLUSION: Simvastatin can promote osteoblastic differentiation and inhibit the proliferation of hBMSC by increasing the expressions of Cbfa1, OSX mRNA.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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