Title: Study of biological characteristics and osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells
Abstract: Objective To isolate and study the phenotype, biological characteristics and osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells( UCBMSC). Methods Mononuclear cells from umbilical cord blood (UCB) were cultured in stem cell medium to obtain mesenchymal stem cells ( MSC). The growth characteristics in vitro was investigated. Immunophenotype and cell cycle were analyzed by FACS. Dexamethasone, ascorbic acid-2-phosphate andβ-glycerophosphate were used to induce osteoblasts and osteogenic potential which were determined by expression of alkaline phosphatase ( ALP), formation of a mineralized extracellular matrix, modulation of osteopontin mRNA expression and typeⅠcollagen expression. Results Human MSCs were successfully isolated from UCB. These cells were adherent with a fibroblast like morphology. Immunophenotyping showed that CD45, CD31,CD11a, CD14 and HLA-DR positive MSCs were 1% -3% , CD166, CD44,CD106,CD29,CD105,CD49e, CD90, CD73 positive MSCs 91% -98%. Cell cycle assay demonstrated 95% of the MSCs being in G0/G1 phase. When the MSCs were induced to osteogenic differentiation, there appeared the positive expression of ALP and typeⅠcollagen, the formation of a mineralized extracellular matrix and the modulation of osteopontin mRNA expression. Conclusion UCBMSC is a source of mesenchymal progenitors and can be induced to osteoblasts in a suitable condition; hence it might be potential seed cell reservoir for bone tissue engineering.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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