Title: The Methodological Study for HPLC Determination of Nifedipine Content
Abstract: A HPLC method for determination of nifedipine content was developed.Chromatographic conditions were as follows:Agilent C18column with temperature of 25℃ was used in separation,mobile phase was methanol-water(60∶40,volume ratio)at the flow rate of 0.8mL·min-1,detection wavelength was 235nm,the injection volume was 20μL.The peak area of nifedipine was in good linear relationship in the range of 12.5~400μg·mL-1,the correlation coefficient was 0.9999.The average of recovery rates with the concentration at high,medium and low were 101.66%,99.99%,99.23%,respectively.The RSD of precision of the determination was 1.56% in day and was 0.79%day-to-day.Nifedipine content of nine bathes nifedipine tablets from different companies were successfully detected out by the method.The results show that the method is exclusive,highly sensitive,accurate,fast,easy and stable for determination of nifedipine content.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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