Title: Mechanism of calpain inhibitor on cardiomyocyte apoptosis induced by high glucose
Abstract:Objective:To investigate the mechanism of calpain inhibitor on cardiomyocyte apoptosis induced by high glucose in cultured rat.Method:Cardiomyocytes were randomly divided into three groups: the contro...Objective:To investigate the mechanism of calpain inhibitor on cardiomyocyte apoptosis induced by high glucose in cultured rat.Method:Cardiomyocytes were randomly divided into three groups: the control group,the high glucose group and the calpain inhibitor(ALLN) group.MTT assay was carried out to detect the viability of cultured cardiomyocytes,and laser confocal microscopy was used to observe the mitochondrial permeable transition and membrane potential.The change of caspase-3 activity in cardiomyocyte was detected by Western blot.Result:The viability of cardiomyocyte was(55±11)% in the high glucose group,and(70±15)% in ALLN group(P0.05).After hyperglycemia,the mitochondrial permeable transition of cardiomyocyte was increased [(30±15)% vs(60±11)%,P0.05] and membrane potential [(22±12)% vs(80±19)%,P0.05] was declined.However,the effects cloud be inhibited by pretreatment of ALLN.Hyperglycemia could increase the expression of cleaved caspase-3,while the expression of cleaved caspase-3 was down-regulated with pretreatment of ALLN [(0.42±0.11) vs(0.21±0.12),P0.05].Conclusion:Calpain inhibitor can protect cardiomyocyte from apoptosis under the high glucose condition.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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