Title: Detection of methylation of the p16 gene in exhaled breath condensate from lung cancer patients
Abstract:Objective:To detect methylation of p16 gene in exhaled breath condensate(EBC)from lung cancer patients,and to assess its potential as a molecular marker for clinical diagnosis of lung cancer.Methods: ...Objective:To detect methylation of p16 gene in exhaled breath condensate(EBC)from lung cancer patients,and to assess its potential as a molecular marker for clinical diagnosis of lung cancer.Methods: With methylation-specific PCR(MS-PCR),the status of methylation of the p16 gene was investigated in EBC from 20 lung cancer patients.Results: Among these 20 EBC specimens,abnormal methylation of p16 gene was found in 8 cases(40%),the positive rates of squamous-cell lung carcinoma,adenocarcinoma and small-cell lung carcinoma were 40.0%,33.3% and 50.0% respectively.Abnormal methylation of the p16 gene in EBC was not significantly correlated with the clinical stage and pathological type of the tumor.But methylation of p16 gene in the EBC was part-methylation.Conclusion: The method of detecting methylation in EBC to diagnose and screen lung cancer showed that p16 gene may not a suitable candidate gene.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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