Title: Effect of Artesunate on Proliferation and Cell Cycle in Human Leukemia K562 Cells
Abstract: Objective:To investigate the effects of artesunate on proliferation and cell cycle in human leukemia K562 cells. Methods:Human leukemia K562 cell lines were treated by artesunate. The cell growth curve was drawn according to cell counts. The inhibitory effect on cell proliferation was assessed by MTT assay. The morphology changes of the nucleons were observed under invert microscope. The level of cell-cycle was determined by flow cytometry (FCM). Results:When the concentrations of artesunate were 1×10-4,1×10-5 and 1×10-6mol/L, the growth of cells was inhibited remarkably in a dose-dependent manner. The IC50 values of artesunate were 3.75×10-6 mol/L. It was seen that cells in different degrees of shrinkage, reduction of nuclear fission, and secretory granules in culture medium under the invert microscope. Flow cytometric analysis showed that cells were increase in G2 phase. Conclusion: Artesunate can inhibit the growth and proliferation effectively in K562 cells. The inhibitory mechanisms on proliferation inhibition may be reducing DNA synthesis and arresting cell cycle progression in G2/M phase.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot