Title: Immune Response Induced by Rabies Vaccine Containing Poly IC Adjuvant in Mice
Abstract: Objective To observe the immune response induced by rabies vaccine containing Poly IC adjuvant in mice. Methods BALB / c mice were divided into three test and two control groups, 15 for each. The mice in three test groups were injected i.p. with diluted hamster kidney cells-based rabies vaccine containing Poly IC adjuvant (PHKCV+P), hamster kidney cells-based rabies vaccine for human use(PHKCV)and national rabies vaccine reference(R)respectively, once on day 0 or twice on days 0 and 7. The mice in one control group(P)were injected with Poly IC adjuvant, while those in the other control group(N)were untreated. Five mice in each group were killed on days 7 and 14 respectively, and their sera were separated for determination of neutralizing antibody. Meanwhile, the spleens were collected for determination of secreted IFNγ levels by ELISPOT method. The rest ten mice in each group were challenged i.c. with CVS strain on days 7 and 14 to observe the protective effect of vaccine. Results The serum neutralizing antibody levels of mice immunized twice with PHKCV+P were significantly higher than those with PHKCV. However, both the specific IFNγ spot formation cell (SFC)counts of mice immunized once and twice with PHKCV+P were significantly higher than those in other groups, while the protective effects were superior to that in PHKCV group. Conclusion Rabies vaccine containing Poly IC adjuvant decreased the amount of antigen used, while enhanced the cellular and humoral immune responses especially early cellular immunity, thus induced higher protective efficacy as compared with traditional rabies vaccine.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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