Title: Mutation breeding of amylase-producing strain Bacillus subtilis by supercritical CO_2
Abstract: Mutation breeding of amylase-producing strain Bacillus subtilis K2 using supercritical CO2 was investigated.Based on the plate method and determination of amylase activity,the mutation conditions including pressure,temperature and time were examined.The optimal mutation conditions were as follows: pressure 8.0 MPa,temperature 37℃,time 30 min.A mutant with almost 33% increase in amylase activity compared with the wild type strain was isolated.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot