Title: Compound mutation of Bacillus subtilis with high-yield of amylase
Abstract: The Bacillus strains preserved in our laboratory was used as the original strain to be mutated using UV mutagenesis and diethyl sulfate in order to increase the production of amylase.A mutant strain which was stable in hereditary was obtained.The results indicated that the strain of D-2 had a high production of amylase.The yield of amylase reached 6.599U/ml,which was 4 folds higher than that of the starting strain.A new efficient starch-hydrolysis enzyme strain screening method was developed,which made a good foundation for further medium optimization.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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