Title: Pathological changes of hepatic stellate cells in rat liver fibrosis induced by one high-dose injection of dimethylnitrosamine
Abstract:Objective To explore the pathological changes of hepatic stellate cells (HSCs) in rat liver fibrosis induced by one high-dose injection of dimethylnitrosamine(DMN). Methods The rats were divided into ...Objective To explore the pathological changes of hepatic stellate cells (HSCs) in rat liver fibrosis induced by one high-dose injection of dimethylnitrosamine(DMN). Methods The rats were divided into control and model groups. Rats were injected intraperitoneally with DMN at one high-dose of 50mg·kg-1 body weight to establish the model. The rats were sacrificed at each of the following post-injection time:hour 6,12,24,36; day 2,3,5,7,10,14;month 1,3,6 and 12. Liver tissues were used for HE staining and immunohistochemical staining of α-smooth muscle actin,ectodermal dysplasia-1(ED-1) and transforming growth factor-β1. Reverse transcription-polymerase chain reaction was used to detect mRNA expression of collagen I and TGF-β1 in liver tissues. Results The pathological characteristics included three stages:early injury-response(6 hours to 5 days),regeneration-fibrosis(5 to 14 days)and perpetuation of fibrosis (14 days to 12 months). At 6h,focal necrosis of liver parenchyma appeared and got the peak of acute submassive hemorrhagic necrosis at 36h; At 3d,HSCs were activated in the residual parenchyma and plenty of macrophages expressing TGF-β1 were infiltrated in the necrotic area;At day 5,HSCs were activated,proliferated and migrated into the necrosis. Some HSCs began to express TGF-β1;At day 7,diffuse activated HSCs were distributed along the sinusoid and in the thin fibrotic septa. At 14d,central-central bridging fibrosis was completely formed. Plenty of activated HSCs were paralleled in the fibrotic septa with macrophages. From then on to 12 mons,the morphology of liver fibrosis was kept without obvious changes. Conclusion The model of rat liver post-necrotic fibrosis by one high-dose injection of DMN was practical. The pathological changes of HSCs,macrophages and hepatocytes,and the interaction among these cells were basis for further studies of liver fibrosis and related liver diseases.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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