Title: Pharmacokinetic of 8-acetylharpagide in Rats
Abstract: Objective: To establish a plasma 8-acetylharpagide in HPLC determination of Khabarovsk to study different routes of administration in rats given 8-acetylharpagide Haba pharmacokinetics after.Method: Rats were injected and orally 8-acetylharpagide,blood was collected at different time points;detected in plasma by HPLC 8-acetylharpagide,acetonitrile-isopropyl alcohol-water(1.8∶10.2∶88),detection wavelength 207 nm,flow rate 1.0 mL · min-1,draw the curve,the establishment of pharmacokinetic models,calculation of pharmacokinetic parameters.Result:Regression equation of 8-acetylharpagide in plasma was Y=613 753.13X-86 995.55(r=0.999 2).In the range of 0.015 3-22.995 μg with good linear relationship.The intra-day and inter-day precision of three dosage were 0.59%,0.38%,0.55%;3.9%,2.1%,1.5% respectively.The extraction recoveries were 94.89%,95.85%,95.25%.The intragastric approach basically can not be detected in thebody;The main pharmacokinetic parameters of injection means by three dosage group were as follows:t1/2(21.15±3.36),(24.26±5.50),(16.58±5.65) min;tmax(4.98±0),(4.98±0),(4.98±0) min;Cmax(2.25±1.41),(0.09±0.03),(0.02±0.007) g·L-1.Conclusion: This method was proved to be sensitive,selective and simple.It can be successfully applied to the pharmacokinetic study of 8-acetylharpagide.The injection given to 8-acetylharpagide in rats in vivoabsorption of Khabarovsk fast and does not completely eliminate relatively slow,but with good pharmacokinetic characteristics,compartment model fitting analysis for the compartment model.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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