Title: Inhibitory effect of celecoxib combined with cisplatin on xenografts of Tca8113 cells in vivo
Abstract: OBJECTIVE:To investigate the inhibitory role of celecoxib combined with cisplatin(DDP) on human tongue squamous cell carcinoma cell line Tca8113 in vivo.METHODS:Nude mice bearing xenografts were treated with as follows:celecoxib,DDP,and celecoxib plus DDP and control.At 35 d after administration,the nude mice were sacrificed and tumor masses were dissected and weighed for calculating tumor inhibition rates.The histological and electron microscopic analyses were used to observe the nude mouse tumor tissues.The immunohistochemistry staining was employed for COX-2 protein expression and fluorescent quantitive real-time RT-PCR was used to detect COX-2 mRNA expression.RESULTS:COX-2 proteins were strongly expressed in the control group while their expressions were significantly decreased in the groups of celecoxib and celecoxib plus DDP.COX-2 inhibitor celecoxib could enhance the inhibition of DDP on the growth of Tca8113 xenograft.The inhibition rates in the celecoxib group,DDP group and celecoxib plus DDP group were 15.63%,37.50% and 82.81%,respectively,which were statistically significant compared with the control group (P0.01).Compared with celecoxib and DDP groups,the inhibitory effect of celecoxib plus DDP was statistically significant (P0.01).The results of RT-PCR showed that celecoxib was less active in inhibiting the expression of COX-2 mRNA (P=0.073).CONCLUSIONS:COX-2 inhibitor celecoxib is capable of enhancing the killing effect of DDP on Tca8113 cells.When co-administered with DDP,the inhibitory effect of DDP on the growth of xenografts in nude mice is significantly enhanced.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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