Title: HPLC fingerprint chromatogram of Semen Cassiae
Abstract:Objective To set up an analysis method of fingerprint chromatogram of Semen Cassiae by RP-HPLC,and lay a basis for the quality control of medical material of Semen Cassiae.Methods RP-HPLC was used on ...Objective To set up an analysis method of fingerprint chromatogram of Semen Cassiae by RP-HPLC,and lay a basis for the quality control of medical material of Semen Cassiae.Methods RP-HPLC was used on a chromatographic column of SunfireTM C18(4.6 mm×150 mm,5 μm),using acetonitrile-phosphoric acid water solution(0.1 %) as the gradient elution,and the mobile phase was at flow rate of 0.8 mL/min.The detection wavelength was at 280 nm and the analysis time was 120 minutes.HPLC fingerprint chromatograms of 10 batches of crude material of Semen Cassiae were analyzed.Results Under the above chromatographic conditions the characteristic peak of fingerprint chromatograms of crude material of Semen Cassiae was composed of 27 chromatographic peaks through similarity analysis.Conclusion The fingerprint chromatogram set up by RP-HPLC is stable and repeatable,which can be used for not only the quality control but also the research of effective principles of Semen Cassiae.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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