Title: Optimization of Extraction Technology of Total Flavonoid from Pulsatilla chinensis by Response Surface Analysis
Abstract: An aqueous two-phase system composed of 21%C2H5OH and 22%(NH4)2SO4was used to extract total flavonoid fromPulsatilla chinensis.The effects of crude extract mass fraction,NaCl mass fraction and pH value on the extraction efficiency of total flavonoid were explored by one-factor-at-a-time and Box-Benhnken design methods.The optimal aqueous two-phase extraction conditions were crude extract mass fraction of 12.5%,NaCl mass fraction of 1.5%and pH of 5.99.Under the optimal extraction conditions,total flavonoid fromPulsatilla chinensis root was mainly distributed in the upper phase with an extraction rate of 73.6%.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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