Title: Optimization of a defined medium for the L-lactic acid production by response surface analysis method
Abstract: Response surface methodology was used to optimize the defined medium for lactic acid production by Lactobacillus paracasei.The influence of eight kind of nutrients including amino acid mixture,nucleosides,vitamins on the cell growth and lactic acid production were investigated by the method of Plackett-Burman,identifying three significant components,that is amino acid mixture,KH2PO4 and CaCO3.The methodology of steepest ascent path was applied to approach the optimal region of the three significant factors.Box-Behnken design and response surface analysis were used to further investigate the optimal central level and the relationship of these factors.The mean value of lactic acid production in three verification experiments was consistent with the predicted value and more than the value in the complex medium.All these results provided a basis for metabolic flux analysis in L.paracasei.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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