Title: EFFECTS OF iNOS-DERIVED NO/ONOO~-ON CASPASE-1 PROTEIN EXPRESSION FOLLOWING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION IN RATS
Abstract: ObjectiveTo investigate the effects of iNOS-derived NO/ONOO~-on Caspase-1 protein expression following focal cerebral ischemia and reperfusion in rats which were sacrificed at 48 h of reperfusion after 2 h of ischemia.MethodsFocal cerebral ischemic model was induced by the occlusion of left middle cerebral artery.Aminoguandine,a selective inhibitor of iNOS,was given intraperitoneally.The content of NO was measured by nitriate reductase method.The expression of nitrotyrosine was examined by flow cytometry.The Caspase-1 expression was detected immunohistochemically.ResultsThe expression of Caspase-1 protein was remarkably lower in aminoguandine groups than vehicle group.ConclusionNO/ONOO~-derived from iNOS might promote apoptosis following focal cerebral ischemia and reperfusion via upregulating Caspase-1 expression.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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