Title: KR3 disease resistance gene transformation in soybean
Abstract: To improve soybean mosaic virus( SMV) resistance,KR3 gene was transformed using Agrobacterium tumefaciens mediated transformation method. Result showed that 12 transgenic soybean plants from 386 transformed explants were identified by coating leaves with glufosinate- ammonium,detecting with Bar protein quick dip stick and PCR analysis. The final soybean transformation efficiency was 3. 11%. RT- PCR results showed that the KR3 expression in transgenic plants was higher than that of non- transgenic plants. 7 days after mechanical SMV inoculation,transgenic plants showed higher resistance. By DAS- ELISA,SMV detection rate was 75% in non-transgenic plants,and was zero among 12 positive transgenic T1 plants. The results suggested that KR3 expression in soybean improved the resistance to SMV.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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