Title: Regeneration of Transgenic Cucumber Plants with a WMV-2 CP Gene
Abstract: Cotyledon segments of Cucumis sativus L. were used as explants for transformation mediated by Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pBPMWMV containing an npt Ⅱgene and a WMV 2 CP gene (Fig.1).Transgenic plants were obtained and segregation of WMV 2 CP gene in R1 transgenic plants was analysed (Table 1). Successful genetic transformation was confirmed by DNA dot hybridization, PCR assay and Southern blot analysis (Plate I). The incidence of virus disease was low and late on transgenic plants (Plate I,Fig.4 and Table 2),thus showing that it had a certain resistance to relevant viruses.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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