Title: Expression of histone deacetylase 3 in acute leukemic cell lines
Abstract: Objective:To study the expression of histone deacetylase 3(HDAC3) in acute leukemic cell lines and to observe whether the HDAC3 expression is necessary for the transcriptional regulation by controlling proliferation and differentiation of leukemia cells.Methods:The HDAC3 mRNA and protein expression levels in 16 acute myeloid leukemic cell lines were assessed by real-time quantitative PCR(RTQ-PCR) and Western blotting,respectively.The mRNA and protein expression levels of HDAC3 were detected during differentiation of NB4 cells induced by all-trans retinoic acid(ATRA).Results:The mRNA expressions of HDAC3 were higher in NB4,NB4RA and NOMO-1 leukemic cell lines but the protein expression levels were not parallel with mRNA expressions.HDAC3 mRNA and protein expression had a decreased tendency during the ATRA-induced differentiation of NB4 cells.Conclusion:HDAC3 is necessary for proliferation of leukemic cells and the expression of HDAC3 in NB4 cell line is associated with cell differentiation and/or proliferation.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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