Title: Effect of methyltransferase on malignant osteosarcoma cells during differentiation inducted in viuro
Abstract: Objective:To observe the change of DNA methyltransferase(Dnmt) in osteosarcoma cell line differentiation inducted in vitro and explore the function of Dnmt and its relation with malignant osteosarcoma cells during differentiation induction in vitro.Methods:After in vitro differentiation inducted by all trans-retinoic acid(ATRA),the changes of morphology and organelles of MG-63 cells were observed by transmission electron microscope.MTT assay was used to study the proliferation and to draw the cell growth curves.Dnmt and PCNA mRNA levels were determined by semi-quantitative RT-PCR.Changes of cell cycle was detected by flow cytometry(FCM).Results:ATRA could significantly inhibit proliferation and induce morphological differentiation of osteosarcoma cells,the value decreased from 0.8405±0.1305 to 0.6147±0.0427(P0.05).The relative expression levels of Dnmt and PCNA mRNA decreased remarkably in osteosarcoma cells with increase of treatment time.Osteosarcoma cell lines accumulated in G_1 phase.Conclusion:ATRA can induce differentiation of human osteosarcoma cells.ATRA may effectively reduce the level of Dnmt through affecting transcription level of PCNA and arresting cell cycle progression.The inhibition of Dnmt mRNA expression in MG-63 cells may be one of the mechanisms for ATRA induced differentiation.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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