Title: Studies on the extraction and antioxidative activity of rosa laevigata michx brown pigment
Abstract: The extraction and antioxidative activity of rosa laevigata michx brown pigment were studied.The results showed that the optimal technological conditions are as follows:temperature 70℃,ratio of solid-liquid 1 ∶6,concentration of ethanol in aqueous solution 60%,time 2 hours.Ultrafiltration membrane and macroporous resin were used to separation and purification.Price of finished products was 143.65(in distilled water).FRAP and DPPH used to evaluate the browm pigment total antioxidative activity and free radical acavenging capacity.The results showed that:the total antioxidative activity was equivalent to 37.11% of BHT,42.01% of vitamin C.The scavenging activity on free radical was equivalent to 67.74% of BHT,50.97% of vitamin C.Fluorescence and chemiluminescence used to determination the clearance of hydroxyl radical and superoxide anion.The results showed that the concentration of 1.0mg/mL,4minutes when the pigment of ·OH and O-2 have better scavenging.Fe2+/linoleic acid test system used to research inhibition of peroxidation of linoleic acid.Laevigata Michx Brown Pigment has a certain inhibitory effect obtained,when the concentration was 1.0mg/mL,83.9% can be achieved.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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