Title: HPLC Analysis of Flavonoids from Reed Leaves
Abstract: Objective: To analyze the compositions of flavonoids from reed leaves.Methods: The crude extract was prepared during the condensation of reed leaf extract.The primarily purified flavonoids were prepared by macro-porous resin.The hydrolysis conditions of flavonoids were optimized.Two samples were hydrolyzed to obtain two hydrolysis products.The compositions of flavonoids were determined by HPLC.Results: The contents of total flavonoids in crude extract and primary purified flavonoids samples were 57.1% and 18.5%,respectively.The optimal hydrolysis conditions for reed leaf flavonoids were hydrolysis temperature of 80-90 ℃,HCl concentration of 1.4 mol/L and hydrolysis time of 4 h.Totally 33 components were separated by HPLC from the crude extract and 8 components were identified based on their retention time.A total of 22 components were separated by HPLC from the primarily purified sample and 5 components were identified.A total of 28 components were separated from the hydrolysates and 7 components were identified.Similarly,25 components were separated from the hydrolyzed primarily purified samples and 7 components were identified.Conclusion: Reed leaves contained rutin,scutellarin,hesperidin,luteolin,quercetin,apigenin,kaempferol,isorhamnetin or hesperetin,isoliquiritigenin,baicalein,and other unknown flavonoids.The content of apigenin among these components was the highest.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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