Title: Effects of Calreticulin on Hippocampus of Post-traumatic Stress Disorder Rats
Abstract: Objective To investigate the expression of molecular chaperone calreticulin(CRT) on the hippocampus in rat post-traumatic stress disorder(PTSD) model,and discuss the regulation of CRT on Ca2+ in hippocampus of PTSD rats,so as to provide an experiment basis for pathogenesis of memory anomaly in PTSD rats.Methods The rat PTSD models were established by using method of single-prolonged stress(SPS).Samples were collected from male Wistar rats before or 1,4,7 days after exposure to SPS.The expression of CRT was detected by using immunohistochemistry,Western blot and RT-PCR.The intracellular free calcium was examined by fluorescence spectrophotometer.Results The expression of CRT in the hippocampus increased after SPS stimulation,and reached the peak at SPS 4 d.The intracellular free calcium level in the hippocampus increased,and reached the peak at SPS 1 d,then gradually decreased.Conclusion PTSD can induce cell apoptosis through endoplasmic reticulum stress,calcium overload,up-regulated expression of CRT,and activation of unfolded protein response,which could be the pathogenesis basis on memory anomaly in PTSD rats.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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