Title: Expressions of Ca~ (2+)-CaM-CaMKIIá signaling in hippocampi of rats with PTSD-like behavior
Abstract: Objective To explore the neurobiological bases in the pathogenesis of the lasting emotional behavioral disorders following posttraumatic stress disorder (PTSD).Methods After establishing the PTSD animal model of subconvulsive stimulation to hippocampus, the intracellular free calcium by Fura-2/AM, free calmodulin (CaM) by flowcytometry, and the total CaM and Ca 2+/CaM dependent kinase II a (CaMKIIa) by Western blotting were detected in hippocampi of experimental rats.Results The intracellular free calcium levels were increased, and the Mean Channel Fluorescence of intracellular free CaM decreased remarkably in 72 h poststimulation, while the expression of total CaM was significantly elevated in 48 h after the last stimulation, and that of CaMKIIa was markedly decreased as well in hippocampi of experimental rats with PTSD-like behavior.Conclusion It indicated that the dysfunction of Ca 2+-CaM-CaMKIIa signaling pathway in hippocampi may play an important role in the long-term neuropsychological sequelae in rats with PTSD-like behavior.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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