Title: Screening to genetic defects of the patients with azoospermia and severe oligospermia
Abstract:Objective:To investigate the relationship between genetic defects and dysgenesis of sperm of patients with azoospermia or severe oligospermia.Methods:G banding techniques were used to conduct the rout...Objective:To investigate the relationship between genetic defects and dysgenesis of sperm of patients with azoospermia or severe oligospermia.Methods:G banding techniques were used to conduct the routine karyotype analysis of peripheral blood chromosome,and polymerase chain reaction(PCR) was used to detect Y-chromosome microdeletion of 36 cases of azoospermia with normal karyotype.Results:The rate of abnormal chromosome karyotype was 30.33%(74 cases) in cases with azoospermia and severe oligospermia and 3.33%(1 case)in control group,respectively.3 cases of Y-chromosome microdeletion in different segments of Azoospermia Factors(AZF) region on Y-chromosome were found in 36 cases of azoospermia with normal karyotype and the microdeletion rate was 8.33%.No microdeletion in corresponding sites was discovered in normal men who had had a child.Conclusion:Both abnormal chromosome karyotype and Y-chromosome microdeletion play important roles in azoospermia and severe oligospermia.It is more accurate and comprehensive in evaluating the genetic defects of patients with azoospermia or severe oligospermia by screening peripheral blood chromosome karyotype with Y-chromosome microdeletion,which can offer the patients with etiologic diagnosis,genetic counseling and choices for therapeutic strategies.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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