Title: Investigating the Effect of Decoction of Xie-zhuo Jie-du on TLR_4,IκB-α and IL-8 in Rats with Ulcerative Colitis
Abstract: Objective: To observe the curative effect of Decoction of Xie-zhuo Jie-du,whose pathogenesis was dispersing turbidity to ascend healthy qi and detoxifying to remove stasis aiming directly at ulcerative colitis,on the expressions of TLR4,IκB-α and IL-8 through animal experiment,and investigate the action mechanism on UC.Method: The animal model of ulcerative colitis was made by trinitro-benzene-sulfonic acid combined with ethanol.All the rats were divided into 5 groups randomly,which were blank group,model group,control group and treatment group of low dose and treatment group of high dose.Then the expressions of TLR4 and IκB-α in colon were detected by immunohistochemistry,and the content of IL-8 in serum was done by radio-immunity.Result: Decoction of Xie-zhuo Jie-du could decrease the expressions of TLR4 and IκB-α markedly.So could the content of IL-8.Conclusion: Decoction of Xie-zhuo Jie-du has good and reliable therapeutical effect on ulcerative colitis,and its mechanism of action may be related to decreasing the expressions of TLR4 and IκB-α and the content of IL-8.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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