Title: Simultaneous Content Determination of Salvianolic acid A,Salvianolic acid B,Tanshinol,Lithospermum Acid,Tanshinone IIA in Crude Extract of Salvia miltiorrhiza Radix et Rhizoma by HPLC
Abstract: Objective:To establish a new RP-HPLC method for simultaneous determination of salvianolic acid A,salvianolic acid B,tanshinol,lithospermum acid,tanshinone ⅡA in crude extract of Radix Salvin Miltiorrhiza.Method: Analysis was carried out on a Kromasil 100-5 C18(4.6 mm×250 mm,5 μm) column with acetonitrile-pure water(added 0.1% formic acid) as mobile phases in gradient elution.The flow rate was 1 mL·min-1,and the UV detector was monitored at 285 nm.Result: All calibration curve showed good linear regression in test ranges(r0.999 5);and the overall recoveries were in the range of 97%-103%(n=3)with RSD less than 3%.The overall RSD of precision test were less than 3%.Conclusion: The method is rapid,accurate,credible and repeatable,and can provide bases for the material basis and compatibility for Radix Salvin Miltiorrhiza.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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