Title: Effects of paclitaxel on the growth and apoptosis induction in Hut78 cell line
Abstract: Objective: To observe the effects of paclitaxel on apoptosis induction in cutaneous T-cell lymphoma cell line Hut78 in vitro. Methods: Hut78 was treated by paclitaxel with different concentration and acting time. Telomerase activity was determined by TRAP-PCR-ELISA. The inhibition of Hut78 cell growth was measured by MTT assay. The apoptosis of Hut78 was examined by flow cytometry. Results: Paclitaxel could down-regulate telomerase activity, inhibit the growth of Hut78, and induce cell apoptosis in a dose and time dependent manner. S phase and G2/M phase DNA content increased and a typical subdiploid peak before G0/G1 phase were observed by flow cytometry. Conclusions: Paclitaxel has obvious effects of growth-inhibiting on cutaneous T-cell lymphoma and can induce apoptosis of Hut78 cell.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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