Title: Cell cycle block and apoptosis induced in esophageal carcinoma cell line by paclitaxel
Abstract: AIM To study paclitaxel-induced cell cycle block and apoptosis in human esophageal carcinoma cells. METHODS Cell morphology,agarose gel electrophoresis and flow cytometry were used to examine paclitaxel-induced cell cycle block and apoptosis in human esophageal carcinoma cell line Eca109. RESULTS Paclitaxel induced cell cycle block at G0/G1 and G2/M phases, and initiated apoptosis with typical morphological feature, apoptotic peak by flow cytometric analysis, cellnant fragmental DNA ladder bands by agarose gel electrophoresis when cells were treated with 3 nmol·L-1 paclitaxel for 72 h. There were different morphological change and cell cycle change between cells treated with 100 nmol· L-1 and 1 000 nmol· L-1 concentration respectively. Additional incubation after removal of paclitaxel which had acted with cells for a short time (<2 h) induced apoptosis earlier than sustained incubation with paclitaxel. CONCLUSION Paclitaxel not only induces mitotic block, but also arrests cells at interphase. Cell cycle block may not be a sufficient signal for paclitaxel-induced apoptosis, and it may prolong the process of apoptosis. There may be several pathways for apoptosis in esophageal carcinoma cells.
Publication Year: 1998
Publication Date: 1998-01-01
Language: en
Type: article
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