Title: Establishment of Agrobacterium-mediated Transformation System of Minghui 63
Abstract: In the study, Agrobacterium-mediated transformation of indica rice was optimized and Minghui 63 was transformed by this system. Results revealed that 133 resistant calluses were obtained from 1100 callus tissues. The transformation efficiency was 12.7% and differentiation efficiency was 81.3%. Besides, segregation ratio of progenies from single-copy transgenic plants fit for Mendal law of 3:1. This suggested that the transformation system was an efficient transformation system and could be used for gene function confirmation, genetic analysis of new genes, and production of marker-free transgenic plants by co-cultivation.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot