Title: Studies on transforming high efficiency insect-resistant gene Pin II into rice
Abstract: One japonica variety and three indica varieties were applied in the genetic transformation of rice by using Agrobacterium tumefaciens LBA4404 harboring binary vector containing Pin Ⅱ and high efficiency promoter ACT I and ACT I intron.The system of high efficiency transformation was established by optimizing many factors involving in tranformation effect,which were methods of callus induction,days of co-culture and adding Ace to medium and etc.Callus derived from mature embryos of rice were infected and co-cultured with Agrobacterium,and many transgenic rice plants were obtained.The highest transformation frequency was 51.8?%(japonica) and 31.5?% (indica),respectively.The ratio of transgenic plants regenerated from selected calli was 67.6?% (japonica) and 47.5?%(indica).The amplified PCR results indicated that insect-resistant gene was integrated into rice genome and the percentage of positive plants was 33.3?% (japonica) and 27.4?% (indica). The putative transformed plants were conformed by insect-resistant assay in field.Pin Ⅱ had effect on transgenic plants because the frequency of destroyed plants by insects was lower than that of its original no-transgenic plants.Many plants,of which agricultural characters were obviously changed,were obtained,and were useful materials for studying its genetic backgrounds and rice breeding.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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Cited By Count: 1
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