Title: Searching for the peptide ligands of β1-adrenergic receptor from phage-displayed peptide library
Abstract: Objective To screen peptide ligands of β1-adrenergic receptor (β1-AR) from phage-displayed peptide library. Methods The synthetic peptide of the second extralleular loop of human β1-AR was used to screen the library with affinity selective method. Positive clones was identified with the methods of enzyme linked immuno-sorbent assay and competitive inhibition test. Results After three round washes, two positive phage clones were selected among 30 clones, in which one clone can interrupt purified polyclonal antibody serum against β1-AR binding to β1-AR. Conclusion The peptide ligand can bind to the target specifically.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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