Title: Detection of apoptosis of the human hepatic carcinoma cells by Annexin V/PI double staining methods
Abstract:Objective To investigate the property of apoptosis of HepG2 cell line induced by CDDP.Methods Liver cancer cell line HepG2 was incubated with CDDP of different concentrations for 4 h,15 h,24 h,36 h,re...Objective To investigate the property of apoptosis of HepG2 cell line induced by CDDP.Methods Liver cancer cell line HepG2 was incubated with CDDP of different concentrations for 4 h,15 h,24 h,36 h,respectively.Apoptosis was measured by flow cytometry method and Annexin V and PI double staining.Results Apoptosis of HepG2 cell could be induced by CDDP,the percentage of AnnexinⅤ+ PI cells increased along with the increase of CDDP concentration and incubation time.Conclusions Apoptosis of HepG2 cell induced by CDDP was time and dose dependent.Annexin V and PI double staining could be a specific method for analyzing apoptotic cells.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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