Title: Effects of GSRb1 on free intracellular calcium concentrations in ischemic neurons of rats
Abstract: Objective To study the effects of the monomers of ginsenoside Rb1 (GSRb1) on the concentrations of intracellular calcium in ischemic neurons of rats.Methods Hippocampus neurons from embryo of rats were cultured in vitro, then placed into normal extracellular fluid (normal control group), simulated ischemic extracellular fluid (ischemia group), simulated ischemic extracellular fluid without calcium (ischemia without calcium group) and simulated ischemic extracellular fluid with different concentration of GSRb1. The fluorescence intensity of intracellular calcium in each group was measured by laser scanning confocal microscope technique, and the corresponding percentage was calculated by comparison with normal control.Results Compared with normal control group, fluorescence intensity of intracellular calcium increased by ( 73.5±10.31)% in ischemia group, ( 4.5±2.58)% in ischemia without calcium group,( 20.2±3.41)%, ( 13.6±2.98)%,( 10.5±3.62)% and ( 12.7±4.51)%, respectively, in simulated ischemic extracellular fluid with different concentration of GSRb1 groups (20, 40, 60 and 80 μmol/L). The decreases of fluorescence intensity of intracellular calcium in simulated ischemic extracellular fluid with different concentration of GSRb1 groups were significantly different compared with ischemia group (all P0.01). Conclusions The increase of intracellular calcium of neurons underwent ischemia/anoxia mainly due to inflow of calcium from extracellular fluid. GSRb1 may play neuroprotective effect by inhibition inflow of calcium and amelioration of calcium overload. This neuroprotective effect is concentration dependent and approaches the most at the concentration of 60 μmol/L of GSRb1.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 1
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