Title: Interstitial calcium and sarcolemal LVDCC expressing in rat myocardial ischemia reperfusion injury in vivo
Abstract: Objective To detect the dynamics of interstitial calcium and sarcolemal LVDCC mRNA expressing in rat myocardial ischemia reperfusion injury(IR)in vivo.MethodsTwelve SD rats were randomized into IR or control groups.Myocardial interstitial fluid was collected continously with microdialysis technique and calcium concentration was measured with an atomic absorption spectrophotometer.In IR group,LAD was ligated for 20 mins then released for 60 mins to achieved IR effects.The left ventricle(ischemic area)and right ventricle was harvested for quantitative PCR of sarcolemal LVDCC at the end.Serum calcium and cTnT were also detected before and after IR.Procedures were the same in control group but no ligating/releasing LAD.ResultsThe cTnT was elevated significantly in IR group[control vs.IR:(1.62±0.60)ng/ml vs.(4.29±2.22)ng/ml,P=0.031].Interstitial calcium concentration kept steady during ischemia,while went down sharply at 20 minutes later in reperfusion.Dialysate calcium concentration of IR group was significantly lower than that of control[control vs.IR:(224.25±31.75)μmol/L vs.(136.25±39.50)μmol/L,P=0.031].There was no difference in both groups for serum calcium before and after experiment.There were no differences in and between groups for sarcolemal LVDCC mRNA expressing.ConclusionsIn this 20 minutes myocardial ischemia and 60 minutes reperfusion injury rat model in vivo,interstitial calcium concentration keeps steady during ischemia,while goes down sharply after reperfusion,which does not correlate with sarcolemal LVDCC mRNA expressing.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot