Title: Neuron protection and expression of c-Jun and c-Fos in neonatal rats after hypoxic-ischemia brain damage and reperfusion
Abstract: AIM: To explore the relationship between c fos and c jun expression and the survival of neurons after the damage of hypoxic ischemia and reperfusion of the brain. METHODS: Seven days old SD rats ( n =56) from sevenbroods were randomly divided into a hypoxic ischemia brain damage (HIBD) group and a sham control group. The HIBD model was established as follows: The right common carotid artery was temporarily occluded for 3 h by an elastic tube with a thread putting through. HIBD group was exposed to 80 mL·L -1 oxygen and 920 mL·L -1 nitrogen gas mixture for 1 h. After 3 h the tube was removed and the common carotid artery was reperfused for different periods. The flow of the blood was detected by the multicolor Doppler. The proteins of c Fos and c Jun were dynamically immunohistochemically evaluated at different reperfusion periods in rat hippocampus. Thionin staining was carried out in the adjacent slices in order to observe the apoptosis and survival of neurons. RESULTS: The right hippocampus in the HIBD group showed obvious positive immunoreactivity against c Fos and c Jun. The number of immunopositive cells reached the peak at 6 h postreperfusion and at 48 h another peak appeared, which was followed by a gradual decline and on d 7 it was the lowest, but, still higher than that in the control group ( P 0.01). The control group showed hardly any positive immunoreactivity. Thionin staining revealed that the apoptosis of the neurons in CA1 region was obvious at 24 h postreperfusion. But on d 7, the loss of neurons was not different from that in the control group ( P 0.05). Similarly, in the CA3 and DG, the neurons remained morphologically intact. The control group showed few apoptosis cells in hippocampus. CONCLUSION: c Fos and c Jun may participate in the repair and survival of hippocampal neurons after hypoxic ischemia reperfusion.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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