Title: Cytotoxicity of PM_(2.5) in type II alveolar epithelial cells(MLE-12)
Abstract: Objective The cytotoxic effect of particulate matters(PM2.5) in type II alveolar epithelial cells(MLE-12) was investigated.Methods In this study,water-soluble and water-insoluble fractions of PM2.5 and PM2.5 suspension were firstly prepared.MLE-12 cells were exposed to different concentrations of water-soluble and water-insoluble fractions of PM2.5 and PM2.5 suspension,respectively.MTT assay was carried out to evaluate the viabilities of PM2.5-treated cells after 24 h exposure.The activities of lactate dehydrogenase(LDH) and catalase(CAT),concentration of glutathione(GSH),and generation of reactive oxygen species(ROS) in MLE-12 cells were determined.Furthermore,the expression of SP-C mRNA in MLE-12 cells was detected by Real-time PCR.Results The viability of MLE-12 cells was significantly decreased after exposure to different fractions of PM2.5 in a dose-dependent manner.The activities of LDH were significantly increased(P 0.01).The generation of H2O2 in water-soluble fraction-treated cells was significantly higher than that in control.However,the generation of H2O2 was significantly decreased after pretreatment with 0.005 mg/ml CAT.The expression of SP-C mRNA in PM2.5-treated cells was lower than that in the control.No significant differences between PM2.5-treated cells and untreated cells were observed after pretreatment with 0.005 mg/ml CAT at the same time.Conclusion The results suggest that water-soluble and water-insoluble fractions of PM2.5 possess cytotoxicity in MLE-12 cells which may be mediated through oxidative stress.Moreover,caution should be taken in the synergistic toxic effect of water-insoluble fraction of PM2.5 in MLE-12 cells.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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