Title: Neuroprotective Effects of SMND-309 on Apoptosis Induced by Oxygen-Glucose Deprivation in SH-SY5Y Cells
Abstract:The human neuroblastoma SH-SY5Y were cultured in a hypoxia/hypoglyc-emia condition to induce cell apoptosis in vitro.The viability of SH-SY5Y cells and level of lactate dehydrogenase(LDH) was measured...The human neuroblastoma SH-SY5Y were cultured in a hypoxia/hypoglyc-emia condition to induce cell apoptosis in vitro.The viability of SH-SY5Y cells and level of lactate dehydrogenase(LDH) was measured by sulfonyl-rhodamine B(SRB) assay and colorimetric,respectively.The characteristic of apoptosis in morphological was observed with Hoechst33258 fluorescence staining,and Western blotting was used to detect the expression of Bcl-2 and Bax.The results in present study suggested that SMND-309 significantly restrained apoptosis of SH-SY5Y cells,degraded the degree of injury and elevated the cellular viability.These effects were correlated to the upregulation of Bcl-2,but not attributed to the change of Bax.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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