Title: HBV Genotypes among Children Carriers with Hepatitis B Virus
Abstract:OBJECTIVE To detect the HBV genotypes among the children carriers of hepatitis B virus in Wenzhou,the distribution of two subtypes of genotype B(Ba and Bj),and the relation of it with viral duplicatio...OBJECTIVE To detect the HBV genotypes among the children carriers of hepatitis B virus in Wenzhou,the distribution of two subtypes of genotype B(Ba and Bj),and the relation of it with viral duplication level.METHODS Real-time fluorescence PCR technology was applied to classify the HBV genotypes and quantify the HBV DNA concentration.For the samples which were determined to genotype B,PCR-RFLP and PCR product sequence analysis were applied to distinguish its two subtypes Ba and Bj.RESULTS The HBV genotypes among the children carriers of hepatitis B virus were classified into B,C,the B+C mixed and D genotypes,accounted for 43.92%,53.44%,1.59% and 1.06%,respectively.There were no significant differences between the level of HBV DNA of genotype C and that of genotype B(P0.05).The 83 samples of HBV genotype B all belonged to subtype Ba,and we had not found subtype Bj.CONCLUSIONS The HBV genotypes among the children carriers of hepatitis B virus in Wenzhou mainly are the genotypes C and B.In two subtypes of genotype B mainly is the subtype Ba.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot