Title: Hypoxia-induced apoptosis and anti-apoptosis in ovarian cancer cell line SKOV-3ipl cells and its correlation with cell cycle
Abstract: Purpose:To investigate hypoxia induced apoptosis and anti apoptosis in ovarian cancer cell line SKOV 3ipl cells and its correlation with cell cycle changes.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an Anaerobic system insulated with 85% N 2,5% CO 2,10% H 2 and very low O 2 concentration (100 ppm). Apoptosis and cell cycles were measured with FITC Annexin V/PI double stain and DNA prep stain Flow cytometry in cells cultured in anaerobic condition. Results:Apoptosis in cells cultured in hypoxia condition for 0h,8h,16h,24h. were (0.32±0.01)%,(8.29±0.87)%,(4.23±0.75)%,(0.59±0.08)% respectively. Between 8h or 16h and 0h, P 0 01. For cell cycles in different time of hypoxia exposure for 0h,8h,16h,24h. G 1 phase were (52.92±1.79)%,(61.88±1.30)%,(78,35±1.42)%,(54.68±1.50)%,S phase were (42.36±2.40)%,(35.98±1.93)%,(11.92±2.05)%,(41.2±1.58)%;G 2 phase were (4.64±0.57)%,(2.12±1.13)%,(11.64±1.62)%,(4.14±0.84)% respectively. In G 1 phase and S phase, between 8h or 16h and 0h, P 0 01; in G1 phase,between 16h and 0h, P 0 01,8h and 0h. P 0.05.After 24hrs hypoxic exposure all cell cycles were the same as that for 0 hr.Conclusions:For ovarian cancer cell line SKOV 3ipl cells, apoptosis and cell cycle arrests were induced during hypoxic exposure for 8h~16h,after 24hrs, anti apoptosis and relief of cell cycle arrests developed. The ability of anti apoptosis and adaptation to hypoxia in SKOV 3ipl cells was not caused by cell cycle arrests.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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