Title: Effect of Bu Yang Huan Wu decoction(补阳还五汤) on basic fibroblast growth factor in rats with cerebral ischemia
Abstract: Objective To investigate the effect of Bu Yang Huan Wu decoction(BYHWD,补阳还五汤) on the expression of basic fibroblast growth factor(bFGF) and its mechanism of anti-cerebral ischemia.Methods Eighty-five Sprague-Dawley rats(body weight 280-300 g) were randomly divided into five groups: normal control group(n=5),sham-operated group,model group(n=20),traditional BYHWD group and ultra-fine powder BYHWD group.Then the latter four groups were further divided into four subgroups of 1,3,5 and 7 days(each n=5).The cerebral ischemic rat model was made by occluding the middle cerebral artery(MCA) with a thread.The traditional decoction and ultra-fine powder decoction groups were ingested with the liquid medicine of BYHWD after 2 hours of the operation(2 ml per rat per day,according to the surface area,it was equivalent to three times the dosage of 70 kg adult(human)).All groups were executed at the 1,3,5 and 7 days after the operation.At the same time there were 5 rats as the normal control group.The brain tissue was taken for the preparation of specimens.The distribution and expression difference of bFGF in the ischemic brain tissues of rats in different groups had been investigated with immuohistochemistry technique.The contents of bFGF in the brain tissue were detected with enzyme linked immunosorbent assay(ELISA).Results The positive expression of bFGF at peri-infarct area after ischemia increased obviously,but at central-infarct area there was less such positive expression.There was no expression at contra-lateral side to the ischemic area.The expression of bFGF immune positive cell increased at 1 day,peaked at 3 days,and returned to baseline at 7 days.The positive cells of bFGF in tradition decoction and ultra-fine powder decoction groups were obviously more than those in the model group at 3,5 and 7 days(all P0.05).The regularity of the change of bFGF protein content in traditional decoction and ultra-fine powder decoction groups was basically the same as that in the model group,and except at 1 day,the contents at all the other time points in the decoction groups were higher than the content in the model group(all P0.05).The comparisons between the traditional decoction and ultra-fine powder groups showed the positive bFGF cell number and its protein content had significant differences at 5 and 7 days(all P0.05).The positive cells and regularity of expression of bFGF in ultrafine powder decoction groups were more than those in tradition decoction at 5 and 7 days(all P0.05).Conclusion ① After brain ischemia,the bFGF positive cell number and its protein content in the brain tissue are increased.② BYHWD can maintain high level expression of bFGF after cerebral ischemia,that might be one of the mechanisms of BYHWD to antagonize the brain injury.③ The therapeutic effect of ultra-fine powder decoction is better than that of tradition decoction.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot