Title: Effects of breviscapine on the renal structure, function and PKC-mRNA and its protein expression in brain-dead BA-Ma mini pigs
Abstract: Objective To investigate the effects of breviscapine on the renal structure, function and PKC-αmRNA and its protein expression in brain-dead BA-Ma mini pigs. Methods Fifteen BA-Ma mini pigs were randomly divided into 3 groups: brain-dead group (group A), breviscapine pretreatment group (group B), and control group (group C), 5 pigs in each group. The brain-dead models were established by increasing intracranial pressure in a modified, slow and intermittent way. At 3, 6, 12, 18 and 24 h after the initial brain death, serum BUN, Cr, TNF-α, IL-1β, and IL-6 were determined. At 3, 6, 12, and 24 h, the changes of renal tissues were observed by HE staining, and the expression of PKC-αmRNA and protein was detected by RT-PCR and immnohistochemistry respectively. The ultrastructural changes of hepatic cells were observed under electron microscopy. Results (1) At 3rd h after the initial brain death, IL-1β, IL-6, and TNF-αin group A and group B began to increase. Serum BUN and Cr in group A and group B began to increase at 12 th after brain death and were higher at each time point (P0. 05). Inflammatory factors at 3, 6, 12, 18, and 24 h had significant differences (P0. 05). BUN and Cr in group B after 12 h were significantly different from those in group A (P0. 05). In group A, these parameters were significantly higher than in group B at each time point (P0. 05). (2) The expression of PKC-αmRNA and protein in groups A and B began to increase at 3rdh. The expression of PKC-αmRNA and protein at 6, 12, and 24 h showed significant differences (P0.05), which was significantly higher in group A than in group B at each time point (P0. 05). (3) After 12 h, changes of renal cells could be found, the injury degree of hepatic cells in group B were milder than that in group A. Conclusion Breviscapine can inhibit the expression of PKC-αmRNA and protein, decrease the release of inflammatory factors, and then alleviate the renal injury during brain death.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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