Title: Effect of Dogwood Polysaccharide on the Expression of Sirt1 Gene in Eye Lens of the Aging Rats
Abstract: OBJECTIVE: To discuss the possible mechanism of the dogwood polysaccharide for treatment of agerelated cataract in an attempt to find new drugs and target points for its prevention and treatment. METHODS:60 Kunming mice were enrolled in our study and divided into 3 groups of 20 each: Control group( C),the aging model group( A) and dogwood polysaccharide administration group( D). In the same environment,Group C were fed with normal diet and subcutaneously injected with 0. 9% Sodium Chloride Injection in the nape daily as per the dosage of D-galactose in the other administration group; Group A and D were also fed with normal diet and subcutaneously injected with D-galactose 100 mg /( kg·d) in the nape daily; after subcutaneous injection for 45 consecutive days,Group C and Group A were fed with warm water at the same dose of drug administration; Group D was fed with dogwood polysaccharide at a dose of 0. 4 g /kg for polysaccharide for 30 consecutive days. At last all mice were killed by cervical dislocation,with eye lens extracted quickly at 4 ℃ and stored in liquid nitrogen for use. T-SOD and T-AOC were detected by immunohistochemical methods,and Sirt1,p53 and FOXO1 mRNA expressions were detected by RT-PCR. RESULTS: Sirt1 mRNA in disease group and the treatment group increased significantly compared with the control group( P 0. 05,P 0. 01),and Sirt1 mRNA in the treatment group increased significantly compared with the disease group( P 0. 01); p53 mRNA in disease group and the treatment group increased significantly compared with the control group( P 0. 01,P 0. 01),but p53 mRNA in the treatment group decreased significantly compared with the disease group( P 0. 05); FOXO1 mRNA in disease group and the treatment group increased significantly compared with the control group( P 0. 05,P 0. 01),and FOXO1 mRNA in the treatment group increased significantly compared with the disease group( P 0. 05). Furthermore,T-SOD and T-AOC in the disease group and the treatment group increased significantly compared with the control group( P 0. 01,P 0. 01),and SOD and T-AOC in the treatment group increased significantly compared with the disease group( P 0. 01,P 0. 01).CONCLUSIONS: Dogwood polysaccharide may regulate the expression of downstream genes p53 and FOXO1 possibly by regulating the expression of Sirt1,ultimately inhibiting or delaying the apoptosis of epithelial cells in eye lens and slow the progression of age-related cataracts.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 1
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