Title: Apoptosis and c-myc expression induced by arsenic trioxide in K562 cells
Abstract:Aim: To investigate the apoptosis and c-myc mRNA expression induced by arsenic trioxide in K562 cells in vitro and to explore its cellular and molecular mechanisms. Methods: Cell proliferation and via...Aim: To investigate the apoptosis and c-myc mRNA expression induced by arsenic trioxide in K562 cells in vitro and to explore its cellular and molecular mechanisms. Methods: Cell proliferation and viability were analyzed by MTT assay and trypan blue exclusion assay. Cell morphological analysis, DNA electrophoresis and flow cytometry were performed to detect whether 0 μmol/L,0.5 μmol/L,1 μmol/L,2 μmol/L,4 μmol/L,8 μmol/L As_2O_3 for 24 h,48 h,and 72 h could induce apoptosis of K562 cell in virto. RT-PCR and immunohistochemistry techniques were used to detect c-myc mRNA and protein expression in K562 cells. Results: As_2O_3 induced chromatin condensation, nuclear fragmentation and the formation of apoptotic body at different concentrations. Cell viability decreased with raised As_2O_3 concentration and prolonged treatment within a certain range. Flow cytometry analysis manifested that As_2O_3 induced K562 cells apoptosis and affected the cell cycle progression. Most of cells were arrested at G_2/M phase. After As_2O_3 treatment c-myc mRNA and protein expression was up-regulated firstly then down-regulated to a lower level. Conclusion: As_2O_3 can inhibit the proliferation and induce apoptosis in K562 cells through blocking the cell cycle at G_2/M phase. The c-myc gene may be involved in the gene regulation of As_2O_3-induced apoptosis.Read More
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot