Title: Effects of dexmedetomidine pretreatment on myocardial injury induced by ischemia-reperfusion of hind limbs in rats
Abstract: Objective To evaluate the effects of dexmedetomidine pretreatment on myocardial injury induced by ischemia-reperfusion(IR)of hind limbs in rats.Methods Thirty healthy SpragueDawley rats,weighing 200-250 g,were randomly divided into 3groups(n=10,each):group sham operation(group S),group IR and group dexmedetomidine pretreatment(group D).Limb ischemia was induced by occlusion of bilateral hind limbs for 4hfollowed by reperfusion for 4hin group IR and group D.Dexmedetomidine 50μg/kg was injected intraperitoneally 30 min before reperfusion of hind limbs in group D,while equal volume of normal saline was injected in group S and group IR.The rats were sacrificed at 4hof reperfusion and myocardial specimens were obtained for microscopic examination of pathologic changes and for determination of superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in myocardial tissues.Left ventricular systolic pressure(LVSP),left ventricular end diastolic pressure(LVEDP) and ± dp/dtmax were measured and recorded.Results Compared with group S,MDA content was significantly increased,and SOD activity was decreased in group IR and D(P0.05).Compared with group IR,MDA content was significantly decreased and SOD activity was increased(P0.05).Compared with group S,LVSP,±dp/dtmax were significantly decreased and LVEDP was increased in group IR and group D(P0.05).Compared with group IR,LVSP,±dp/dtmax were significantly increased and LVEDP was decreased in group D(P0.05).The pathological changes were significantly attenuated in group D compared with group IR.Conclusion Dexmedetomidine pretreatment can reduce myocardial injury induced by IR of hind limbs in rats and the mechanism may be related to inhibition of lipid peroxidation and increasein the blood flow of myocardium.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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