Title: Effects of Ligustrazine on Expressions of Matrix Metalloproteinase-9,Tissue Inhibitor of Metalloproteinase-1 and Cytokines in Rats with Intestinal Adhesion Tissues
Abstract: Objective:To determine the expressions of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the tissues of intestinal adhesion and cytokine interleukin 1(IL-1),tumor necrosis factor alpha(TNF-α) and transformation growth facto(rTGF-β) in blood sera of rats,in order to explain the mechanism of ligustrazine on intestinal adhesion.Methods:Seventy-two SD male rats were randomly assigned to 4 groups:normal group,sham operation group,operation group,and drug group,18 rats in each group.Traumatic model of intestinal adhesion model was established in the operation and drug group.In the sham operation group,the anesthesia and laparotomy were the same as the operation group,but no intestinal tissue was injured and no mesenteric artery was clamped.In the drug group,rats were intraperitonealiy injected with 10 mL of ligustrazine solution(about 0.1 g ligustrazine),while in the rest groups equivalent physiological saline was injected.In the four groups,6 rats were respectively sacrificed on day 2,7,and 14 after modeling,and tissues of serous membrane in adhesive area were taken.Under macroscopic observation,intestinal adhesion were classified.Elisa method was used for determination of cell factor IL-1,TNF-α,and TGF-β.The expressions of MMP-9 and TIMP-1 in the intestinal adhesive tissues and the normal intestinal tissues were determined by immunohistochemistry assay and image analysis.Results:(1)Compared with the sham operation and normal groups,the intestinal adhesion degree in the operation and drug group significantly increased(P0.05);and compared with the operation group,the drug group had lower degree of interstinal adhesion(P0.05).(2)Compared with the operation group,the levels of IL-1,TNF-αand
TGF-β in the drug group significantly decreased,with statistically significant difference(P0.05 or P0.01).(3) Compared with the operation group,the MMP-9 expression reduced on day 2 after modeling,but the TIMP-1 expression did not increase to a significantly different level until day 7(all P0.01).On day 7 and 14,the drug group had the expressions of TIMP-1 increased(P0.05),but did not have a significant increase in MMP-9.Conclusions:Ligustrazine can inhibit intestinal adhesion in rats by reducing IL-1,TNF-α,and TGF-β and regulating MMP-9 and TIMP-1 in the intestinal tissues.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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