Title: Effects of Qingrezaoshi decoction on the expression of MMP-2, TIMP-2 and their mRNA in ALI rats caused by LPS.
Abstract: Objective To investigate the effects of Qingrezaoshi decoction on the expression of MMP-2 and their mRNA(MMP-2 mRNA),tissue inhibitor of MMP-2 and its mRNA(TIMP-2 mRNA) in the rats with acute lung injury(ALI) caused by LPS.Methods Forty male Wistar rats were randomly divided into 4 groups:normal control group,LPS model group,dexamethasone group and Qingrezaoshi decoction group.Each group had two subgroups,4 h and 8 h after LPS injection.All rats,except normal control group,were administrated with LPS by intravenous injection to induce ALI.The rats in dexamethasone group and Qingrezaoshi decoction group were treated by dexamethasone(oral) and Qingrezaoshi decoction(oral) respectively before LPS-induced ALI.The expression of MMP-2 and TIMP-2 were measured by immunohistochemistry ABC,and their mRNAs were measured by real-time fluorescent PCR,while the histopathology of the lung injury was observed by light microscope.Results In 4 h Qingrezaoshi decoction group,the expression of MMP-2 mRNA was significantly decreased while the expression of TIMP-2 protein and its mRNA were significantly increased compared with model group(P 0.01 or P 0.05).In 8 h Qingrezaoshi decoction group,the expression of MMP-2 protein and its mRNA were significantly decreased(P 0.01 or P 0.05).Pathological observation showed that pulmonary hemorrhage and necrosis were largely seen in model group and a better condition in Qingrezaoshi decoction group.Conclusion Qingrezaoshi decoction may abate the histopathology of the lung injury and it has protective effects on endotoxin-induced ALI in rats.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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